Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells

Authors

  • Abbas Ghaderi Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran - Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Amin Ramezani Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran - Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
  • Elham Mahmoudi Maymand Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran.
  • Fatemeh Sadat Toghraie Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran.
  • Mahsa Yazdanpanah-Samani Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran.
  • Seyedeh Masoumeh Sharifzadeh Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical science, Shiraz, Iran - Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract:

Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected with both circular and linear forms of the pBud-hIL-7 recombinant by electroporation. Expression of rhIL-7 in CHO-K1 cells was confirmed by enzyme-linked immunosorbent assay (ELISA) and dot and western blots. Results: On western blots of transformed cells, a single 25 kDa band was observed, consistent with the expected molecular weight of glycosylated hIL-7. No significant expression difference was observed between cells transfected with circular or linear plasmids. Conclusions: We established a stable CHO-K1 cell line expressing rhIL-7, which we consider to be a promising system for the production of rhIL-7 as a biopharmaceutical.

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Journal title

volume 6  issue 1

pages  66- 73

publication date 2017-10

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